AXIN2 and colonic neoplasm: To further assess Wnt upregulation, we performed qRT-PCR analysis using total RNA isolated from the colonic tumors of APCMin and APC-Cldn1 and detected increased mRNA expression of the established Wnt-target genes CD44 (p = 0.0159), Lgr5 (5-fold increase), and Axin2 (3-fold increase) (Figure 3C).