To confirm in vivo the therapeutic effects of hepatocyte targeting expression rIL-10 on liver fibrosis, in vitro we examined the expression of procollagen type I and α-SMA in HSCs co-cultured with BRL cells transfected with rIL-10 expression plasmid pcDNA3-rIL-10, null plasmid pcDNA3 or saline. The gene discussed is ACTA1; the disease is Hepatic fibrosis.