PLEKHA3 and infection: Consistent with data in Dictyostelium, we observed localization of GFP-FAPP1 to vacuoles containing wild type L. pneumophila in mammalian cells at 1 hour post-infection, but not vacuoles containing a ΔdotA mutant (Figure S7A); and GFP-FAPP1R18L, which is deficient in PI4P binding [53], was not observed in association with LCVs (Figure S7B).