PRKN and Parkinson disease: We observed comparable levels of parkin expression and pulldown across all conditions for WT and R275W mutant, while the W453X mutation was not well expressed or IPed (Figure 5b), as described earlier.35, 36 We observed that WT Bax interacted with WT parkin, and that the PD-linked loss-of-function mutant R275W pulled down more WT Bax despite a comparable parkin IP (Figure 5b), as previously described.3 However, the interaction between parkin and Bax was lost in all cases when the BH3 domain of Bax was replaced (Figure 5b).