In conclusion, the most relevant finding of the present work was the development of calcification in the skeletal muscle of hind legs, exclusively with Querétaro strain and particularly during the acute phase of infection, even though both strains studied belong to DTU I. This calcification event may probably be a consequence of the exacerbated inflammatory process and the consequent disruption of the tissue elicited by this infection and could be related to a significant increment in TNF-α levels. Here, TNF is linked to infection.