Mild positivity of immunoreaction (tryptase, CD15, IL-1β, IL-6, IL-8, TNF-α) and stronger reactivity for IL-15, MCP-1 in areas where depletion of cellular antigens (myoglobin and cardiac troponin) is detectable within 30 – 40 minutes from ischemia. The gene discussed is IL1B; the disease is ischemia.