qRT-PCR data showed that all PDAC lines exhibited significantly higher (up to 8.1-folds) levels of AEG-1 mRNA compared to the normal pancreatic ductal epithelial cells, while Western blot analysis showed that AEG-1 protein was highly expressed in all pancreatic cancer cell lines including AsPC-1, Mia Paca-2, and Panc-1. This evidence concerns the gene MTDH and familial pancreatic carcinoma.