To confirm that GSK3A inhibition re-sensitize drug-resistant colon carcinoma cells to chemotherapy, we then treated three cell lines characterized by different genetic background, HCT116p53KO, SW480 and HT-29 drug-resistant cells, with 5FU in the presence of 20 μM SB216763 and 1 μM BIO (Fig. 3). This evidence concerns the gene GSK3A and colon carcinoma.