We have recently demonstrated that pretreatment with the P1A1 antagonist DPCPX alleviate the symptoms to CYP-induced cystitis [31], and the aims of the current study were thus to examine if muscarinic receptors and NO are also involved in the inflammation in experimental CYP-induced cystitis, as well as to investigate if there exists a link between the two. This evidence concerns the gene PPIG and chronic cystitis.