Then, we will ask several important questions in this study: (1) what are the roles of miR-143 in tumor growth and angiogenesis; (2) what is the potential direct target of miR-143 that may be associated with cancer development; and (3) whether miR-143 overexpression inhibits AKT, ERK1/2, and NF-κB signaling pathways via its direct target; (4) What role of miR-143 and underlying mechanisms in glioma resistance to TMZ treatment. The gene discussed is MAPK3; the disease is glioma.