Characteristics of 1321N1, SW1783 and LN18 glioma cells undergoing apoptosis while treated with combined bioactives were determined by the presence of active caspase-3 and the staining of exposed phosphatidylserine on the cell surface which is demonstrated during annexin-V FITC/PI flow cytometry assay. The gene discussed is ANXA5; the disease is central nervous system cancer.