In the present study, we confirmed these findings using MCF-7/LTED and HCC-1428/LTED cells and also found that low dose fulvestrant (2 nM) markedly down-regulated ER expression (Supplementary Fig. S1A) and suppressed proliferation dose-dependently in both parental and LTED MCF-7 and HCC1428 cells, although response of LTED cells is lower than that of parental controls (Supplementary Fig. S1 B, C). The gene discussed is ESR1; the disease is hepatocellular carcinoma.