In the discovery cohort multivariate analyses adopting an additive model adjusted for gender, age at biopsy, BMI, T2DM and PNPLA3 rs738409 genotype found that TM6SF2 rs58542926 (β=0.549±0.135, 95%CI 0.285–0.813; P=5.57 × 10−5) and NCAN rs2228603 (β=0.419±0.138, 95%CI 0.148–0.689; P=0.0026) were both significantly associated with stage of fibrosis. This evidence concerns the gene NCAN and type 2 diabetes mellitus.