Knockdown of MMP-2 in MDA-MB-231 cells (Figure 5A) and MCF10A cells (Additional file 1: Figure S7), or treatment of the cells with MMP inhibitor GM6001 (data not shown), dramatically impacted Gα12QL-stimulated cell invasion These results clearly demonstrated that MMP-2 plays a major role in breast cancer cell invasion stimulated by G12 activation, confirming previous observations with MCF10A cells [14].To assess the importance of the enhanced secretion of IL-6 and IL-8 in cancer cell invasion stimulated by Gα12QL, we neutralized these secreted cytokines using monoclonal antibodies. Here, CXCL8 is linked to breast carcinoma.