To confirm that miR-184 is directly regulated by C-Jun, we first used siRNA [23] to suppress the expression of C-Jun in lung cancer A549 and SPCA1 cell lines as confirmed by Western blot (Fig. 7A).The expression of miR-184 was markedly increased by qPCR analysis in both cell lines (Fig. 7B). The gene discussed is ATP2C1; the disease is lung carcinoma.