In addition, there were no significant changes during the stepwise progression of breast cancer (from normal breast to ADH/FEA, Ptrend = 0.284; from ADH/FEA to DCIS, Ptrend = 0.929; from DCIS to IBC, Ptrend = 0.096), although Alu methylation tended to decrease during the transition from DCIS to IBC (Figure 1A). The gene discussed is AVP; the disease is breast cancer.