In this study, bFGF was used to stimulate the cell cycle progression in HCC cell lines, hSulf-1 was re-expressed by adenoviral vector to block the bFGF-mediated signaling pathways, and the regulatory effects and mechanisms of hSulf-1 on HCC cell cycle control were investigated in the in vitro experiments. This evidence concerns the gene FGF2 and hepatocellular carcinoma.