Additional comparative analysis of modified gene levels across three different CRC cell lines (HT-29, SW480 and LoVo) indicated that silibinin significantly and consistently mediates its effect by down regulation of NANOG, NEUROD1, SOX-2, DACH1, EGR3, POU4F1 and PPARG genes, while at the same time, up regulating TERT levels. The gene discussed is TERT; the disease is colorectal carcinoma.