For this, we isolated different cell populations (CD44+EpCAMhigh, CD44+EpCAMlow, CD44−EpCAMhigh and CD44−EpCAMlow) from human CRC cell lines SW480, HT29 and LoVo (Fig.1A) and subjected them to sphere cluster formation assay to determine which of the isolated fractions was enriched in CSC population. Here, CD44 is linked to colorectal carcinoma.