CCL2 and Familial prostate cancer: Furthermore, qPCR analysis revealed no change in CXCL12 (Fig S2A) or CCL2 (Fig S2B) mRNA expression following stimulation with rh-CXCL8 in any of the prostate cancer cell lines studied; moreover, we observed that the secretion of either CCL2 or CXCL12 from any of the prostate cancer cells was below the limits of detection of the ELISA assays employed.