We isolated RD cell lysates that were either infected with EV71 at a MOI of 40 for 4 h or without infection, and then a Prp8 antibody was applied to pull-down the Prp8/RNA or Prp8-3Dpol/RNA complexes in the lysates, named RNA-binding protein IP (RIP) assay. The gene discussed is PRPF8; the disease is infection.