SPRY4 and cancer: Louis, MO, USA) to evaluate cancer cell invasion, and found that invasion of SPC-A1, A549, and H1299 cells was reduced by 52% following upregulation of SPRY4-IT1 expression, while increased SPRY4-IT1 expression levels impeded the migration of all three cell types by approximately 65% compared with controls, as shown by Transwell assays (Figure 5b and Supplementary Figure S3c).