Independent of the signaling pathways that are employed or the effects that chemicals or even complex mixtures of natural products may exhibit to cause oxidative stress, it was shown here that HSP70B' expression and the derived reporter gene output serve as a valuable detection setup that enabled the construction of a rapid and sensitive in vitro pre-screening test for oxidative stress dysregulation, which is a fundamental characteristic that appears early in the onset of human skin disorders. Here, HSPA6 is linked to skin disorder.