Studies targeting Tmprss6 in Hbbth3/+ and Hfe knockout mice by injecting silencing RNA (Schmidt et al., 2013) and anti-sense oligonucleotides (Guo et al., 2013) have successfully suppressed Tmprss6 mRNA expression, leading to elevated hepcidin levels, improved iron overload in Hfe knockout and anemia and β-thalassemic mice. Here, HFE is linked to anemia (phenotype).