Interestingly, analysis of the lymphocyte cell responses from AML samples demonstrated that; 1) as expected lymphocytes did not respond to the myeloid growth factors and lacked CyclinA2 expression and; 2) in agreement with the results from analysis of gated AML cells, etoposide induced a robust p-H2AX response in CyclinA2- lymphocytes while treatment with TMZ, PARPi or PARPi + TMZ had minimal effects on CyclinA2- lymphocytes (Figure 8). The gene discussed is H2AX; the disease is acute myeloid leukemia.