To test the ability of SCNP to measure cell-cycle specific DDR responses to specific genotoxic agents in primary samples, peripheral blood or bone marrow samples from patients with AML were stimulated to proliferate by 48 h incubation with a panel of myeloid growth factors then evaluated for p-H2AX induction after 6 h in vitro treatment with etoposide, PARPi, TMZ, or the combination of PARPi + TMZ. The gene discussed is H2AX; the disease is acute myeloid leukemia.