In the case of MM, changes in CCN1 mRNA splicing patterns appear independent of pH change and oxygen deficiency, nor is it mediated by soluble factors, as indirect co-culture with MSC using trans-well inserts, as well as by incubating with MSC-derived conditioned media, was not sufficient to induce CCN1 splicing in INA-6 cells. This evidence concerns the gene CCN1 and Miyoshi myopathy.