Our qRT-PCR analyses revealed that the mRNA expression levels of MAGEA-3, MAGEA-1, p16, and p15 were significantly increased in the hepatocellular carcinoma HepG2 cell lines after treatment with 10 nM DAC for 72 h and that the RASSF1A and BRCA1 levels were unchanged (Figure 5(a)). This evidence concerns the gene RASSF1 and hepatocellular carcinoma.