GAST and colorectal carcinoma: To determine whether gastrins stimulate PAK1 activity in CRC cells, DLD1 cells were stimulated with Gamide or Ggly for 10 min in the presence or absence of LY294002, a PI3K inhibitor, and the activity of PAK1 was determined by measuring the ratio of the phosphorylated and active form of PAK1 to total PAK1 by Western blot.