To determine whether gastrins stimulate PAK1 activity in CRC cells, DLD1 cells were stimulated with Gamide or Ggly for 10 min in the presence or absence of LY294002, a PI3K inhibitor, and the activity of PAK1 was determined by measuring the ratio of the phosphorylated and active form of PAK1 to total PAK1 by Western blot. The gene discussed is PAK1; the disease is colorectal carcinoma.