Here, we have further demonstrated that PAK1 was activated in human CRC cell lines in vitro by both Gamide and Ggly through PI3K‐dependent pathways, and that the activation of PAK1 contributed to gastrin‐stimulated activation of β‐catenin signaling and VEGF production in CRC cells, as PAK1 knockdown completely blocked these stimulatory effects of gastrins on CRC cells. Here, PAK1 is linked to colorectal carcinoma.