GLI1 and colon carcinoma: Of interest, the predicted binding constants (KD) for GANT61-diamine-GLI1 (7.5 μM) or GANT61 inhibition of GLI-DNA binding (3.2 μM) are consistent with GANT61 concentrations of 10 μM-20 μM for 48 hr-72 hr exposures, required to induce cell death in human colon carcinoma cell lines [36-38].