In healthy controls, low-dose insulin infusion (20 mU/m2/min) significantly suppressed the rate of interstitial glycerol release (Basal: 286 ± 40 vs. low-dose insulin: 143 ± 18 μmol/l·h; p < 0.001), whereas it did not suppress release in NASH subjects (Basal: 383 ± 44 vs. low-dose insulin: 379 ± 43 μmol/l·h; p > 0.05). Here, INS is linked to metabolic dysfunction-associated steatohepatitis.