To evaluate the effect of PCs inhibition by Spn4A on the ability of colon cancer cells to colonize the liver, HT-29/CTL, CT-26/CTL, HT-29/Spn4A, and CT-26/Spn4A cells, were injected in mice through the intrasplenic/portal route. This evidence concerns the gene DDX53 and malignant colon neoplasm.