NPC cells were treated with ATP (100 μM) for the indicated time, and the cells were then subjected to immunofluorescence and western blotting, as shown in Figure 4A. ATP was found to significantly decrease the expression of cytosolic and nuclear p65 in both the 5-8 F and CNE-2 cells, similar to the situation for p65, and ATP also decreased the expression of OPN in the two NPC cell lines (Figure 4B). This evidence concerns the gene SPP1 and nasopharyngeal carcinoma.