In this study an attempt was made to understand the molecular mechanism involved in Hsp90 mediated regulation of CHIKV infection in mammalian cells using CHIKV prototype strain (S 27) and Indian outbreak strain of 2006 (DRDE-06) as we reported earlier that the 2006 Indian outbreak strain exhibits different pattern of infection in comparison to the prototype strain [38]. The gene discussed is HSP90AA1; the disease is infection.