MMP2 and cancer: Additionally, the kinetics of enzymatic hydrolysis of 5FAM-THPs (MMP-2 hydrolysis of 5FAM6-THP kcat/kM was 1.5 × 104 M−1 s−1) were well within the range of kinetic parameters of enzymatic probes that have successfully visualized proteolytic activity in vivo. Finally, the gelatinases secreted from HT-1090 human cancer cells were capable of proteolyzing 5FAM6-THP, which was confirmed by an increase in fluorescence signal resulting from THP hydrolysis and dequenching of 5FAM fluorophores.