In addition, it was found that the overexpression of Sirt1 is associated with a high TNM classification (P=0.002) (Fig. 3), particularly in lymph node invasion (P=0.018) (Fig. 4) and metastasis (P=0.048) (Fig. 5), but not primary tumor (P=0.442) and tumor size (P=0.151). This evidence concerns the gene SIRT1 and neoplasm.