The main findings of present study are as follows: i) the majority of VGSC α subunits can be detected in NP and BPH samples; however, Nav1.6/Nav1.7 in NP and BPH samples exhibit very low expression levels compared with those in prostate cancer cells; ii) in prostate cancer (PC-3 and LNCaP) cells, the expression of Nav1.6 and Nav1.7 is dramatically upregulated; and iii) the upregulated Nav1.6 and Nav1.7 α subunits in PC-3 cancer cells are functional. Here, SCN8A is linked to pachyonychia congenita.