RC- and HF-fed Nrf2−/− livers had much reduced mRNA levels for Cpt1a, which is required for β-oxidation of long-chain FA, compared to RC-fed Nrf2+/+ livers, with the HF diet reducing Cpt1a mRNA in Nrf2+/+ livers to a much lesser extent than in Nrf2−/− livers (Fig. 4D). This evidence concerns the gene NFE2L2 and hydrops fetalis.