Immunoblotting for the nuclear nonhistone protein HMGB1 in cytosolic fractions also suggested release in RC- and HF-fed Nrf2−/− livers of nuclear protein into the cytoplasm, which suggests loss of nuclear membrane integrity in a significant portion of hepatocytes that lack Nrf2 (Fig. 8F). The gene discussed is HMGB1; the disease is hydrops fetalis.