Western blotting revealed that the active form of Srebp-1 (encoded by Srebf1) was most abundant in the nuclei of HF-fed Nrf2−/− livers but was also elevated in the nuclei of RC-fed Nrf2−/− and HF-fed Nrf2+/+ livers compared with RC-fed Nrf2+/+ livers (Fig. 4B). Here, NFE2L2 is linked to hydrops fetalis.