Next, we infected CD3/CD28 stimulated CD4+ T-cells with wt JR-CSF, S142N or the K421D Env pseudotyped virus, and assessed the infection of the indicated CD4+ T-cell subsets (Figure 4B) via intracellular p24 staining and multiparametric FACS analysis three days post-infection. This evidence concerns the gene CD4 and infection.