Stable GGR Affinofile cell lines with optimal properties were single cell cloned as described in methods.To determine the ability of GGR Affinofile cells to detect HIV-1 infection, we infected a stable clone of GGR Affinofile cells (at maximum CD4/CCR5 induction) using a range of viral inoculums (JR-CSF, MOI = 0.5 – 0.0625) and serially sampled the infected cell culture supernatant for GLuc activity. This evidence concerns the gene CD4 and HIV-1 infection.