To determine whether overexpression of catalytically active MEK with D-site mutations would result in an infection phenotype similar to that induced by PD98059 inhibition of MEK [21], we allowed 3–5 d old female mosquitoes transformed for midgut-specific overexpression of wtMEK, pMEK2 or pMEK5 to feed on P. berghei-infected mice for 30 min (Figure 6). The gene discussed is MAP2K7; the disease is infection.