To examine whether CD24+ cells have intrinsic stem cell properties, we analyzed the expression of five embryonic stem cell genes (Sox2[27], Oct4[28]–[30], Nanog[31], Bmi-1[32], and Rex-1[33]) in TW02 and TW04 cell lines which were used as representative NPC cells. Here, POU5F1 is linked to nasopharyngeal carcinoma.