To differentiate between the FIP200 and WIPI2 binding requirements for Atg12–5-16L1 recruitment to the phagophore during Salmonella infection, we transfected Atg16L1Δ/Δ MEFs with WT FLAG-Atg16L1, ERER (WIPI2-binding mutant), or DRDR (FIP200-binding mutant) (Figure 7G). Here, ATG16L1 is linked to Salmonella Infections.