As shown in Table 1, H4K20me3 status was not associated with pathological classification such as ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC), or more detailed classification of IDC[24], but it was negatively correlated with nuclear grade (P <0.05) and MIB-1 index (P <0.01). The gene discussed is MIB1; the disease is ductal breast carcinoma in situ.