Based on the real-time PCR, expression levels of BIM mRNA were similarly silenced in all stable pancreatic cancer cell lines containing shBIM (Fig. 10B), These experimental clones and control clone (one clone from scrambled shRNA - shCON) were treated with HNA (1, 10 uM), and cell viability were determined by MTT assays. This evidence concerns the gene BCL2L11 and familial pancreatic carcinoma.