AGER and cancer: In order to isolate the impact of RAGE in response to rS100A4, and to mimic the up-regulation of RAGE in cancer tissue, we ectopically overexpressed RAGE in HCT116, SW620 and DLD-1 cells.HCT116/RAGE cells showed a 12-fold increase of RAGE mRNA expression, compared to the control cell lines HCT116 and HCT116/vector.