Consistent with the results of exogenously added HGF, HGF-transfected H2228 (H2228/HGF) cells became insensitive to alectinib (Fig. 2A), indicating that endogenously-expressed HGF also induced resistance to alectinib in EML4-ALK NSCLC cells. This evidence concerns the gene ALK and non-small cell lung carcinoma.