The findings that identified proteins are enriched for cytoskeletal regulators combined with the finding that NMMHCIIA is highly expressed in podocyte major processes led us to test whether NMMHCIIA expression was necessary for the formation of the podocyte cytoskeleton in vitro. Additionally, a cardinal feature of podocytopathy in the setting of HIVAN is a transition from Rho to Rac-mediated arrangement of the actin cytoskeleton [45], [46]. The gene discussed is AKT1; the disease is HIV-associated nephropathy.