On the 21st day, a set of biochemical parameters were studied: the tumor weight and tumor volume, the inhibition rate of lung metastasis, the percentage and ratio of spleen CD4+IL-17+ Th17 (T helper cell 17, Th17 for short) and CD4+CD25+Foxp3+ Treg (T regulatory cell, Treg for short) cells, and the concentrations of IL-6, TGF-β, IL-17, IL-23, and IFN-γ in culture supernatants of CD4+ T lymphocytes were determined. Here, TGFB1 is linked to neoplasm.