Figure 8 illustrates sepsis decreased IGF-I mRNA in both male and female mice, but the reduction was greater (P<0.05) in both WT and DKO female mice. Elevations in inflammatory cytokines can contribute to muscle catabolism and inhibit mTOR [41]. In male mice, sepsis only increased muscle TNFα mRNA in DKO mice, not WT animals (Figure 9A), whereas sepsis increased TNFα in both genotypes of female mice (Figure 9B). A sepsis-induced increase in muscle IL-6 mRNA was detected in both male and female WT and DKO mice (Figure 9C and 9D), with the increase being greater in male DKO mice. This evidence concerns the gene IGF1 and Sepsis.