At days 0, 14, and 28, a number of cytokine (IL-8, IL-1β, IL-6, IL-10, TNF-α, and IL-12p70) and chemokine (RANTES, MIG, MCP-1, IP-10, eotaxin-1, and GM-CSF) were analyzed by using CBA to compare levels in wash samples of the apical surfaces of NP (n = 9) and control NM (n = 7) ALI cultures. This evidence concerns the gene IL1B and acute respiratory distress syndrome.