Therefore, the correlation between FDG uptake and the hypoxia markers, Glut-1, HIF-1α, PI3K and Akt, was investigated in the present study using immunohistochemistry to clarify whether FDG-PET can be used to predict tumor hypoxia and the prognosis of patients with laryngeal carcinoma. This evidence concerns the gene AKT1 and laryngeal carcinoma.