FZD8 and acute myeloid leukemia: Since the Wnt signaling genes Fzd5 and Fzd8 showed enhanced CGI hypermethylation with increasing disease stage (Figure 2B, C), we examined the methylation state of the homologous human CGI sequences in sets of MDS (15.7% and 8.8% median DNA methylation for FZD5 and FZD8, respectively) and normal karyotype AML (42.5% and 15.5%) patient samples.